De Villota is the surname of two people relevant to Formula One:
- Emilio de Villota (born 1946), Spanish racing driver who drove in F1 from 1976 to 1982, mostly for private entries. He entered fifteen races but only started two. Father of María de Villota and Emilo de Villota, Jr., who did not reach F1.
- María de Villota (1980–2013), Spanish female racing driver who tested for Renault and Marussia in 2011 and 2012. Suffered a horrendous accident testing in 2012 and was found dead in a Seville hotel room in 2013. Daughter of Emilio de Villota.